Investigating Science 11–12 · Year 11
Growth of radish seedlings watered with saline water
Module 2: Cause and Effect – Inferences and Generalisations
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The idea
Plant growth falls once the salinity of the water a crop receives passes a threshold, and a class trial infers that threshold from its own data before checking it against published secondary data.
What you need
- 60 radish seeds (Raphanus sativus), 15 pots of 10 cm diameter, seed-raising mix
- sodium chloride and tap water, five labelled 2 L bottles, conductivity meter reading in mS/cm (1 mS/cm = 1 dS/m)
- balance to 0.01 g, ruler, measuring cylinder, gloves and dust mask for handling potting mix
How to do it
- Make five irrigation solutions: tap water only (record its reading), and tap water with salt added until the meter reads 1.0, 2.0, 3.5 and 6.0 dS/m.
- Sow four seeds per pot, three pots per solution, and thin to two seedlings per pot after germination.
- Water every pot with the same volume of its solution on the same days for 4 weeks.
- Harvest, rinse and blot the plants; record shoot height and fresh mass of shoot and root.
- Express each treatment’s mean mass as a percentage of the tap-water treatment and plot it against conductivity.
- Compare the class curve with the FAO crop salt-tolerance data for radish and write the inference and its limits.
What you should see
FAO Irrigation and Drainage Paper 29 (Table 4) gives radish yield potential against irrigation-water salinity (ECw) of 100 % up to 0.8 dS/m, 90 % at 1.3, 75 % at 2.1, 50 % at 3.4 and 0 % at 5.9 dS/m. Four-week pot seedlings are not field yields, so the class curve is compared with the shape of the FAO relation, which predicts 96 % at 1.0 dS/m, 76 % at 2.0, 47 % at 3.5 and no yield at 6.0 dS/m. The learner reports percentage growth with the range across the three pots and names the differences between the trial and the field data that limit the generalisation.
What changes
- What you change
- salinity of irrigation water (dS/m)
- What you measure
- fresh mass of shoots and roots after 4 weeks, as a percentage of the tap-water treatment
- What you keep the same
- same mix and pot size
- same watering volume and schedule
- same light and temperature
- two seedlings per pot
Common misconceptions
Each of these ideas is wrong, and the activity is a chance to test it.
- A little salt always helps plants grow.
- If seeds germinate the water is safe for the crop.
- One pot per treatment is enough to draw a conclusion.
Safety card
Hazards
- potting and seed-raising mixes can carry Legionella bacteria
- wet floors
Controls
- dampen mix before use to keep dust down
- wear gloves and a dust mask when handling mix
- wash hands afterwards
Note
No hazardous chemicals or heat. Potting mix is a biological hazard: record the handling controls in the school’s RiskAssess risk assessment.
Curriculum references
The NSW syllabus outcomes and Australian Curriculum v9 codes this activity supports. They are references, not a verified or complete curriculum alignment.
- Investigating Science Stage 6 Syllabus (2017), NESA; currentINS11-9INS11/12-1INS11/12-2INS11/12-4
- Earth and Environmental Science Stage 6 Syllabus (2017), NESA; current, replaced by the 11–12 Syllabus (2025) from 2028EES11-11
- Australian Curriculum v9No Australian Curriculum v9 code is listed.
Sources
The pages the author read to write this activity.
- www.nsw.gov.au/education-and-training/nesa/curriculum/science/investigating-science-stage-6-2017
- www.nsw.gov.au/education-and-training/nesa/curriculum/science/earth-and-environmental-science-stage-6-2017
- www.fao.org/4/t0234e/T0234E03.htm
- www.fao.org/4/t0234e/T0234E01.htm
- www.soilquality.org.au/factsheets/salinity-nsw
- www.saps.org.uk/teaching-resources/resources/216/investigating-if-plants-grow-better-with-fertiliser